Evidence that BmTXKβ–BmKCT cDNA from Chinese scorpion Buthus martensii Karsch is an artifact generated in the reverse transcription process

@article{Zeng2002EvidenceTB,
  title={Evidence that BmTXK$\beta$–BmKCT cDNA from Chinese scorpion Buthus martensii Karsch is an artifact generated in the reverse transcription process},
  author={Xian-Chun Zeng and San-Xia Wang},
  journal={FEBS Letters},
  year={2002},
  volume={520},
  url={https://api.semanticscholar.org/CorpusID:24619868}
}
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28 Citations
Background Citations
5
Methods Citations
2

28 Citations

Characterization of a novel cDNA encoding a short venom peptide derived from venom gland of scorpion Buthus martensii Karsch: Trans-splicing may play an important role in the diversification of scorpion venom peptides

Template switching generated during reverse transcription?

Reverse transcriptase template switching and false alternative transcripts.

Expression pattern analysis of transcribed HERV sequences is complicated by ex vivo recombination

High resolution sequence analysis suggested that HERV-KX sequences were produced by recombination, possibly arising ex vivo, between transcripts from different HML-2 proviral loci, which should be interpreted very cautiously.

Characterization of BmKbpp, a multifunctional peptide from the Chinese scorpion Mesobuthus martensii Karsch: Gaining insight into a new mechanism for the functional diversification of scorpion venom peptides

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Apparent Non-Canonical Trans-Splicing Is Generated by Reverse Transcriptase In Vitro

It is proposed that most reported examples of non-canonical splicing in metazoans arise through template switching by reverse transcriptase during cDNA preparation, providing a simple diagnostic for identifying many of these experimental artifacts.

When good transcripts go bad: artifactual RT-PCR 'splicing' and genome analysis.

The underappreciated phenomenon of "template switching", by which reverse transcriptase may create artifactual splicing-like events in the preparation of cDNA/EST libraries, may cause complications in searches for newly gained introns in repetitive regions.

Hsp90n - An accidental product of a fortuitous chromosomal translocation rather than a regular Hsp90 family member of human proteome.

Absence of the JAZF1/SUZ12 chimeric transcript in the immortalized non-neoplastic endometrial stromal cell line T HESCs.

The presence, if any, of a JUZF1/SUZ12 chimeric transcript in the immortalized normal T HESCs is not a constant, reproducible result.

6 References

A naturally occurring non‐coding fusion transcript derived from scorpion venom gland: implication for the regulation of scorpion toxin gene expression

Intergenic mRNA Molecules Resulting fromtrans-Splicing*

It is shown that mRNA production may not necessarily be limited to single genes, as human liver also has the potential to produce a variety of hybrid cytochrome P450 3A mRNA molecules.

Cloning and characterization of a cDNA sequence encoding the precursor of a chlorotoxin-like peptide from the Chinese scorpion Buthus martensii Karsch.

Temperature-dependent template switching during in vitro cDNA synthesis by the AMV-reverse transcriptase.

During in vitro cDNA synthesis, the avian myeloblastosis virus (AMV) reverse transcriptase can switch from one template to another in a homology-dependent and temperature-dependent manner.

Molecular cloning and characterization of four scorpion K(+)-toxin-like peptides: a new subfamily of venom peptides (alpha-KTx14) and genomic analysis of a member.

Molecular cloning and sequencing of two ‘short chain’ and two ‘long chain’ K+ channel‐blocking peptides from the Chinese scorpion Buthus martensii Karsch