Successful Transplantation of In Vitro Expanded Human Cadaver Corneal Endothelial Precursor Cells On to a Cadaver Bovine’s Eye Using a Nanocomposite Gel Sheet

@article{Parikumar2014SuccessfulTO,
  title={Successful Transplantation of In Vitro Expanded Human Cadaver Corneal Endothelial Precursor Cells On to a Cadaver Bovine’s Eye Using a Nanocomposite Gel Sheet},
  author={Periyasamy Parikumar and Kazutoshi Haraguchi and Akira Ohbayashi and Rajappa Senthilkumar and Samuel J. K. Abraham},
  journal={Current Eye Research},
  year={2014},
  volume={39},
  pages={522 - 526},
  url={https://api.semanticscholar.org/CorpusID:23131826}
}
The use of the NC gel sheet makes HCEP cell transplantation feasible for human patients, and further in vitro basic studies followed by translational studies are necessary to bring this method for clinical application in appropriate indications.
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16 Citations
Background Citations
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Methods Citations
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16 Citations

Human corneal endothelial cell transplantation using nanocomposite gel sheet in bullous keratopathy.

Transplantation of in vitro expanded human corneal endothelial precursors (HCEP) cells using a nanocomposite (D25-NC) gel sheet as supporting material in bovine's cornea to three patients with bullous keratopathy and visual acuity improved in one of the patients by post-operative day 3 and was maintained at 18 months follow-up.

In Vitro Evaluation and Transplantation of Human Corneal Endothelial Cells Cultured on Biocompatible Carriers

Test in vitro the surgical potential of cultivated human corneal endothelial cells on human anterior lens capsule, LinkCell™ bioengineered collagen sheets of 20-µm thickness (LK20), and denuded Descemet membrane (dDM) as tissue-engineered grafts for DesceMet membrane (DM) endothelial keratoplasty (DMEK) to bypass the problem of donor tissue availability.

A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells

TGP reconstituted with MK and Optisol—GS media yields better preservation of human corneal buttons in terms of relatively higher ECD maintenance and better in vitro culture outcome of corneAL limbal tissue.

Evaluation of the Suitability of Biocompatible Carriers as Artificial Transplants Using Cultured Porcine Corneal Endothelial Cells

In vitro DMEK surgery showed HALC as the most suitable carrier for cultivated pCECs with good intraoperative graft handling and LK20 carrier showed good biocompatibility, but required a DSEK-adapted surgical protocol.

Experimental models of corneal endothelial cell therapy and translational challenges to clinical practice.

Corneal endothelium tissue engineering: An evolution of signaling molecules, cells, and scaffolds toward 3D bioprinting and cell sheets

Different corneal endothelium regeneration methods are compared, the importance of three‐dimensional bioprinting strategies and simulation of Descemet's membrane by biomimetic topography are discussed, and the recent advances, applications, and prospects of cell sheet engineering for CETE are dissected.

New developments in corneal endothelial cell replacement

The description of aspects involved in the isolation of hCEC from donor tissue is described, the different natural and bioengineered carriers currently used in endothelial cell sheet transplantation are described, and the current 'state of the art' in novel therapeutic approaches such as endothelialcell injection are discussed.

Establishment of an Ex Vivo Human Corneal Endothelium Wound Model

The ex vivo model established in this study can provide an alternative to the animal model in studying corneal endothelium decompensation and provide an alternative to the animal model in studying corneal endothelium decompensation.

Translational issues for human corneal endothelial tissue engineering

The aim of this review article is to provide an update of the translational issues currently facing human corneal endothelial cell therapy, and to consider together whether a cell‐based, tissue‐engineering approach is now much closer to attaining success in the treatment of cornea endothelial diseases via a cell- based, tissue-engineering approach.

Scaffold-free and scaffold-based cellular strategies and opportunities for cornea tissue engineering

Limits in current corneal treatment are outlined and the self-healing potential of each cornea cellular layer is highlighted to frame necessities for cell-based bioengineering strategies.

8 References

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