Next-generation sequencing transforms today's biology

@article{Schuster2008NextgenerationST,
  title={Next-generation sequencing transforms today's biology},
  author={Stephan C. Schuster},
  journal={Nature Methods},
  year={2008},
  volume={5},
  pages={16-18},
  url={https://api.semanticscholar.org/CorpusID:1465786}
}
A new generation of non-Sanger-based sequencing technologies has delivered on its promise of sequencing DNA at unprecedented speed, thereby enabling impressive scientific achievements and novel…
View on Nature
ncbi.nlm.nih.gov
1,762 Citations
Highly Influential Citations
45
Background Citations
621
Methods Citations
220
Results Citations
2

1,762 Citations

Application of 'next-generation' sequencing technologies to microbial genetics

The capabilities of high-throughput sequencing technologies are reviewed and the many options for getting useful information from the data are discussed.

Challenges in the Next-Generation Sequencing Field

This chapter compares next-generation sequencing with the traditional Sanger method to demonstrate the remarkable recent developments in relation to improved speed, throughput, and accuracy.

Sequential sequencing by synthesis and the next-generation sequencing revolution.

Next-generation sequencing: applications beyond genomes

A snapshot of these exciting new approaches to understanding the properties and functions of genomes is provided, given that sequencing-based assays may increasingly supersede microarray-basedAssays, to compare and contrast data obtained from these distinct approaches.

First- and Next-Generations Sequencing Methods

This chapter provides an overview of the majority of sequencing methods including techniques from the first- and next-generation of DNA sequencing.

Integrating Next Generation Sequencing, Bioinformatics and Cytogenomics in the Study of Brazilian Mammals

Since the discovery of the DNA double helix, understanding the complexity and diversity of genomes has been a major focus of genetics, including medical and evolutionary research. Sequencing methods…

Development of Next Generation Sequencing Protocols for dsRNA Viruses

Next-generation sequencing methodologies is now changing the way that the authors understand viruses, particularly in the areas of genome sequencing, evolution, ecology, discovery and transcriptomics, and expect new challenges to understand the role of newly discovered viral diversity in both disease and health.

Whole Genome Sequencing in the Clinical Laboratory

The opportunities and challenges of developing a MPS protocol for whole genome sequencing within a clinical laboratory as well as its potential applications for clinical use are discussed.

Next generation sequencing: chemistry, technology and applications.

High-throughput next generation sequencing has been quickly adapted into many aspects of biomedical research and begun to engage with the clinical practice, but significant huddles need to be overcome before an ultimate application of NGS in genomic medicine can be practical and fruitful.

The emergence of nanopores in next-generation sequencing

This review summarizes past, present, and future DNA sequencing techniques, which are realized by nanopore approaches such as those pursued by Oxford Nanopore Technologies.
...

19 References

A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes.

This study evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730xl Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone.

Materials and Methods Som Text Figs. S1 and S2 Tables S1 to S4 References Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome

A prototype of a DNA sequencing technology in which a commonly available, inexpensive epifluorescence microscope is converted to rapid nonelectrophoretic DNA sequencing automation and a savings in metabolic energy is described.

Genome sequencing in microfabricated high-density picolitre reactors

A scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophoresis instruments with 96% coverage at 99.96% accuracy in one run of the machine is described.

DNA sequencing with chain-terminating inhibitors.

A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.

A new method for sequencing DNA.

Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described.

Whole-Genome Shotgun Sequencing of Mitochondria from Ancient Hair Shafts

The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 14C years old and another had been stored for 200 years at room temperature.

Metagenomics to Paleogenomics: Large-Scale Sequencing of Mammoth DNA

The high percentage of endogenous DNA recoverable from this single mammoth would allow for completion of its genome, unleashing the field of paleogenomics.

Nucleotide sequence of bacteriophage φX174 DNA

The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs.

Comprehensive mutation identification in an evolved bacterial cooperator and its cheating ancestor.

In insight into the genetic basis of two large adaptive transitions in a social bacterium, a single mutation responsible for the restoration of development in strain PX was identified, whereas 14 mutations occurred during the prior phase of experimental evolution.

Analysis of one million base pairs of Neanderthal DNA

A 38,000-year-old Neanderthal fossil that is exceptionally free of contamination from modern human DNA is identified and it is revealed that modern human and Neanderthal DNA sequences diverged on average about 500,000 years ago.